1RNG

SOLUTION STRUCTURE OF THE CUUG HAIRPIN: A NOVEL RNA TETRALOOP MOTIF

Summary for 1RNG

• Entry DOI: 10.2210/pdb1rng/pdb
• Descriptor: RNA (5'-R(*GP*GP*CP*GP*CP*UP*UP*GP*CP*GP*UP*C)-3') (1 entity in total)
• Functional Keywords: rna, tetraloop
• Total number of polymer chains: 1
• Total formula weight: 3820.30

Authors

Jucker, F.M.,Pardi, A. (deposition date: 1995-09-05, release date: 1995-12-07, Last modification date: 2024-05-22)

Primary citation

Jucker, F.M.,Pardi, A.
Solution structure of the CUUG hairpin loop: a novel RNA tetraloop motif.
Biochemistry, 34:14416-14427, 1995

PubMed Abstract: The solution structure of a uniformly 13C/15N-labeled CUUG RNA hairpin loop has been determined by multidimensional heteronuclear magnetic resonance spectroscopy in combination with distance geometry and restrained molecular dynamics calculations. The structure of this CUUG tetraloop represents a novel RNA loop motif where the first and last loop nucleotides form a standard Watson-Crick C-G base pair and the second loop nucleotide interacts directly with the closing base pair of the stem by folding into the minor groove. This structure helps explain why the closing base pair is phylogenetically conserved and indicates a six-nucleotide G(CUNG)C motif for the CUUG RNA tetraloop. Implications for the function of this CUUG tetraloop in ribosomal RNA and in RNA tertiary interactions are discussed.

PubMed: 7578046
DOI: 10.1021/bi00044a019

Experimental method

SOLUTION NMR