1RK8

Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex

1RK8 の概要

• エントリーDOI: 10.2210/pdb1rk8/pdb
• 関連するPDBエントリー: 1HL6 1oo0 1P27
• 分子名称: CG8781-PA protein, Mago nashi protein, Within the bgcn gene intron protein, ... (5 entities in total)
• 機能のキーワード: mrna processing; rrm; rbd; nmd; oskar mrna localization, translation
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• 細胞内の位置: Nucleus: Q9V535 P49028; Cytoplasm: P82804
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 43107.66

構造登録者

Bono, F.,Ebert, J.,Guettler, T.,Izaurralde, E.,Conti, E. (登録日: 2003-11-21, 公開日: 2004-04-13, 最終更新日: 2024-02-14)

主引用文献

Bono, F.,Ebert, J.,Unterholzner, L.,Guettler, T.,Izaurralde, E.,Conti, E.
Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex
EMBO Rep., 5:304-310, 2004

PubMed Abstract: The exon junction complex (EJC) is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. The Mago-Y14 heterodimer is a core component of the EJC. Recently, the protein PYM has been identified as an interacting partner of Mago-Y14. Here we show that PYM is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. PYM interacts directly with Mago-Y14 by means of its N-terminal domain. The crystal structure of the Drosophila ternary complex at 1.9 A resolution reveals that PYM binds Mago and Y14 simultaneously, capping their heterodimerization interface at conserved surface residues. Formation of this ternary complex is also observed with the human proteins. Mago residues involved in the interaction with PYM have been implicated in nonsense-mediated mRNA decay (NMD). Consistently, human PYM is active in NMD tethering assays. Together, these data suggest a role for PYM in NMD.

PubMed: 14968132
DOI: 10.1038/sj.embor.7400091

実験手法

X-RAY DIFFRACTION (1.9 Å)