1R8O
Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds
Summary for 1R8O
| Entry DOI | 10.2210/pdb1r8o/pdb |
| Related | 1R8N |
| Descriptor | Kunitz trypsin inhibitor (3 entities in total) |
| Functional Keywords | kunitz (sti) trypsin inhibitor, beta-trefoil fold, copaifera langsdorffii, hydrolase inhibitor |
| Biological source | Copaifera langsdorffii More |
| Total number of polymer chains | 2 |
| Total formula weight | 18031.78 |
| Authors | Krauchenco, S.,Nagem, R.A.P.,da Silva, J.A.,Marangoni, S.,Polikarpov, I. (deposition date: 2003-10-27, release date: 2004-05-25, Last modification date: 2024-10-30) |
| Primary citation | Krauchenco, S.,Nagem, R.A.P.,Da Silva, J.A.,Marangoni, S.,Polikarpov, I. Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii. Biochimie, 86:167-172, 2004 Cited by PubMed Abstract: The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed. PubMed: 15134830DOI: 10.1016/j.biochi.2004.03.004 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.83 Å) |
Structure validation
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