1R8H

Comparison of the structure and DNA binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus

1R8H の概要

• エントリーDOI: 10.2210/pdb1r8h/pdb
• 分子名称: Regulatory protein E2, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: anti-parallel beta-barrel, dna-binding domain, transcription, replication
• 由来する生物種: Human papillomavirus type 6a
• 細胞内の位置: Host nucleus: Q84294
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 62774.72

構造登録者

Dell, G.,Wilkinson, K.W.,Tranter, R.,Parish, J.,Brady, R.L.,Gaston, K. (登録日: 2003-10-24, 公開日: 2003-12-23, 最終更新日: 2024-10-30)

主引用文献

Dell, G.,Wilkinson, K.W.,Tranter, R.,Parish, J.,Leo Brady, R.,Gaston, K.
Comparison of the structure and DNA-binding properties of the E2 proteins from an oncogenic and a non-oncogenic human papillomavirus.
J.Mol.Biol., 334:979-991, 2003

PubMed Abstract: Human papillomaviruses (HPVS) that infect the genital tract can be divided into two groups: high-risk HPV types, such as HPV 16 and HPV 18, are associated with cancer, low-risk HPV types, such as HPV 6, are associated with benign warts. In both high-risk and low-risk HPV types, the papillomavirus E2 protein binds to four sites within the viral long control region (LCR) and regulates viral gene expression. Here, we present the crystal structure of the minimal DNA-binding domain (DBD) from the HPV 6 E2 protein. We show that the HPV 6 E2 DBD is structurally more similar to the HPV 18 and bovine papillomavirus type 1 (BPV1) E2 proteins than it is to the HPV 16 E2 protein. Using gel retardation assays, we show that the hierarchy of E2 sites within the HPV 16 and HPV 6 LCRs are different. However, despite these differences in structure and site preference, both the HPV 16 and 6 E2 DBDs recognise an extended version of the consensus E2 binding site derived from studies of the BPV1 E2 protein. In both cases, the preferred binding site is 5'AACCGN(4)CGGTT3', where the additional flanking base-pairs are in bold and N(4) represents a four base-pair central spacer. Both of these HPV proteins bind preferentially to E2 sites that contain an A:T-rich central spacer. We show that the preference for an A:T-rich central spacer is due, at least in part, to the need to adopt a DNA conformation that facilitates protein contacts with the flanking base-pairs.

PubMed: 14643661
DOI: 10.1016/j.jmb.2003.10.009

実験手法

X-RAY DIFFRACTION (1.9 Å)