1R4I

Crystal Structure of Androgen Receptor DNA-Binding Domain Bound to a Direct Repeat Response Element

1R4I の概要

• エントリーDOI: 10.2210/pdb1r4i/pdb
• 分子名称: 5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*TP*CP*AP*AP*GP*AP*AP*CP*AP*G)-3', 5'-D(*CP*TP*GP*TP*TP*CP*TP*TP*GP*AP*TP*GP*TP*TP*CP*TP*GP*G)-3', Androgen receptor, ... (4 entities in total)
• 機能のキーワード: ar, steroid receptor, protein-dna complex, androgen, transcription-dna complex, transcription/dna
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Nucleus: P15207
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34589.54

構造登録者

Shaffer, P.L.,Jivan, A.,Dollins, D.E.,Claessens, F.,Gewirth, D.T. (登録日: 2003-10-06, 公開日: 2004-06-29, 最終更新日: 2024-10-16)

主引用文献

Shaffer, P.L.,Jivan, A.,Dollins, D.E.,Claessens, F.,Gewirth, D.T.
Structural basis of androgen receptor binding to selective androgen response elements.
Proc.Natl.Acad.Sci.USA, 101:4758-4763, 2004

PubMed Abstract: Steroid receptors bind as dimers to a degenerate set of response elements containing inverted repeats of a hexameric half-site separated by 3 bp of spacer (IR3). Naturally occurring selective androgen response elements have recently been identified that resemble direct repeats of the hexameric half-site (ADR3). The 3D crystal structure of the androgen receptor (AR) DNA-binding domain bound to a selective ADR3 reveals an unexpected head-to-head arrangement of the two protomers rather than the expected head-to-tail arrangement seen in nuclear receptors bound to response elements of similar geometry. Compared with the glucocorticoid receptor, the DNA-binding domain dimer interface of the AR has additional interactions that stabilize the AR dimer and increase the affinity for nonconsensus response elements. This increased interfacial stability compared with the other steroid receptors may account for the selective binding of AR to ADR3 response elements.

PubMed: 15037741
DOI: 10.1073/pnas.0401123101

実験手法

X-RAY DIFFRACTION (3.1 Å)