1R02
Solution structure of Human Orexin-A:Regulator of Appetite and Wakefulness
Summary for 1R02
Entry DOI | 10.2210/pdb1r02/pdb |
Related | 1CQ0 |
NMR Information | BMRB: 5994 |
Descriptor | Orexin-A (1 entity in total) |
Functional Keywords | turn, helix-loop-helix, neuropeptide |
Biological source | Homo sapiens (human) |
Total number of polymer chains | 1 |
Total formula weight | 3588.19 |
Authors | Kim, H.-Y.,Hong, E.,Kim, J.-I.,Lee, W. (deposition date: 2003-09-19, release date: 2004-09-19, Last modification date: 2024-10-23) |
Primary citation | Kim, H.Y.,Hong, E.,Kim, J.I.,Lee, W. Solution structure of human orexin-A: regulator of appetite and wakefulness. J.Biochem.Mol.Biol., 37:565-573, 2004 Cited by PubMed Abstract: Orexin-A and orexin-B (hypocretin-1 and hypocretin-2, respectively) are important hypothalamic neuro-peptides, which are encoded by a single mRNA transcript and stimulate food intake as well as regulate wakefulness. Here we determined the solution structure of orexin-A by NMR spectroscopy and by simulated-annealing calculation. The structural features of orexin-A involve two alpha-helices, with the hydrophobic residues disposed to on one side of helix, and hydrophilic residues to the other. A hydrophilic turn induced by two disulfide bonds provides the key difference between orexin-A and -B. With previous mutagenic studies, the derived structure of orexin-A provides us with a structure-functional view for novel drug design. PubMed: 15479620DOI: 10.5483/BMBRep.2004.37.5.565 PDB entries with the same primary citation |
Experimental method | SOLUTION NMR |
Structure validation
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