1QXB

NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9

Summary for 1QXB

• Entry DOI: 10.2210/pdb1qxb/pdb
• Descriptor: 5'-d(CpGpCpGpApApTpTpCpGpCpG)-3', beta-D-ribofuranose (2 entities in total)
• Functional Keywords: disaccharide nucleotide, backbone modification, b-helix, ribose, dna
• Total number of polymer chains: 2
• Total formula weight: 7627.04

Authors

Nauwelaerts, K.,Vastmans, K.,Froeyen, M.,Kempeneers, V.,Rozenski, J.,Rosemeyer, H.,Van Aerschot, A.,Busson, R.,Efimtseva, E.,Mikhailov, S.,Lescrinier, E.,Herdewijn, P. (deposition date: 2003-09-05, release date: 2004-02-03, Last modification date: 2024-05-01)

Primary citation

Nauwelaerts, K.,Vastmans, K.,Froeyen, M.,Kempeneers, V.,Rozenski, J.,Rosemeyer, H.,Van Aerschot, A.,Busson, R.,Lacey, J.C.,Efimtseva, E.,Mikhailov, S.,Lescrinier, E.,Herdewijn, P.
Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.
Nucleic Acids Res., 31:6758-6769, 2003

PubMed Abstract: A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.

PubMed: 14627809
DOI: 10.1093/nar/gkg911

Experimental method

SOLUTION NMR