1QVA

YEAST INITIATION FACTOR 4A N-TERMINAL DOMAIN

1QVA の概要

• エントリーDOI: 10.2210/pdb1qva/pdb
• 分子名称: INITIATION FACTOR 4A (2 entities in total)
• 機能のキーワード: rna helicase, dead box, eif4a, gene regulation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm: P10081
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24949.70

構造登録者

Johnson, E.R.,McKay, D.B. (登録日: 1999-07-07, 公開日: 1999-12-02, 最終更新日: 2024-02-14)

主引用文献

Johnson, E.R.,McKay, D.B.
Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase
RNA, 5:1526-1534, 1999

PubMed Abstract: The eukaryotic translation initiation factor 4A (elF4A) is a representative of the DEAD-box RNA helicase protein family. We have solved the crystallographic structure of the amino-terminal domain (residues 1-223) of yeast elF4A. The domain is built around a core scaffold, a parallel alpha-beta motif with five beta strands, that is found in other RNA and DNA helicases, as well as in the RecA protein. The amino acid sequence motifs that are conserved within the helicase family are localized to the beta strand-->alpha helix junctions within the core. The core of the amino terminal domain of elF4A is amplified with additional structural elements that differ from those of other helicases. The phosphate binding loop (the Walker A motif) is in an unusual closed conformation. The crystallographic structure reveals specific interactions between amino acid residues of the phosphate binding loop, the DEAD motif, and the SAT motif, whose alteration is known to impair coupling between the ATPase cycle and the RNA unwinding activity of elF4A.

PubMed: 10606264
DOI: 10.1017/S1355838299991410

実験手法

X-RAY DIFFRACTION (2.5 Å)