1QQT

METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI

1QQT の概要

• エントリーDOI: 10.2210/pdb1qqt/pdb
• 分子名称: METHIONYL-TRNA SYNTHETASE, ZINC ION (3 entities in total)
• 機能のキーワード: rossmann fold, helix bundle, trna ligase, ligase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P00959
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 62908.43

構造登録者

Mechulam, Y.,Schmitt, E.,Maveyraud, L.,Zelwer, C.,Nureki, O.,Yokoyama, S.,Konno, M.,Blanquet, S. (登録日: 1999-06-08, 公開日: 2000-01-01, 最終更新日: 2024-02-14)

主引用文献

Mechulam, Y.,Schmitt, E.,Maveyraud, L.,Zelwer, C.,Nureki, O.,Yokoyama, S.,Konno, M.,Blanquet, S.
Crystal structure of Escherichia coli methionyl-tRNA synthetase highlights species-specific features.
J.Mol.Biol., 294:1287-1297, 1999

PubMed Abstract: The 3D structure of monomeric C-truncated Escherichia coli methionyl-tRNA synthetase, a class 1 aminoacyl-tRNA synthetase, has been solved at 2.0 A resolution. Remarkably, the polypeptide connecting the two halves of the Rossmann fold exposes two identical knuckles related by a 2-fold axis but with zinc in the distal knuckle only. Examination of available MetRS orthologs reveals four classes according to the number and zinc content of the putative knuckles. Extreme cases are exemplified by the MetRS of eucaryotic or archaeal origin, where two knuckles and two metal ions are expected, and by the mitochondrial enzymes, which are predicted to have one knuckle without metal ion.

PubMed: 10600385
DOI: 10.1006/jmbi.1999.3339

実験手法

X-RAY DIFFRACTION (2.03 Å)