1QOH

A MUTANT SHIGA-LIKE TOXIN IIE

1QOH の概要

• エントリーDOI: 10.2210/pdb1qoh/pdb
• 関連するPDBエントリー: 1BOS 1BOV 2BOS
• 分子名称: SHIGA-LIKE TOXIN IIE B SUBUNIT (2 entities in total)
• 機能のキーワード: toxin, receptor binding, protein-carbohydrate recognition, specificity
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 151808.22

構造登録者

Pannu, N.S.,Boodhoo, A.,Armstrong, G.D.,Clark, C.G.,Brunton, J.L.,Read, R.J. (登録日: 1999-11-08, 公開日: 2000-07-03, 最終更新日: 2024-11-13)

主引用文献

Ling, H.,Pannu, N.S.,Boodhoo, A.,Armstrong, G.D.,Clark, C.G.,Brunton, J.L.,Read, R.J.
A Mutant Shiga-Like Toxin Iie Bound to its Receptor Gb(3): Structure of a Group II Shiga-Like Toxin with Altered Binding Specificity
Structure, 8:253-, 2000

PubMed Abstract: Shiga-like toxins (SLTs) are produced by the pathogenic strains of Escherichia coli that cause hemorrhagic colitis and hemolytic uremic syndrome. These diseases in humans are generally associated with group II family members (SLT-II and SLT-IIc), whereas SLT-IIe (pig edema toxin) is central to edema disease of swine. The pentameric B-subunit component of the majority of family members binds to the cell-surface glycolipid globotriaosyl ceramide (Gb(3)), but globotetraosyl ceramide (Gb(4)) is the preferred receptor for SLT-IIe. A double-mutant of the SLT-IIe B subunit that reverses two sequence differences from SLT-II (GT3; Gln65-->Glu, Lys67-->Gln, SLT-I numbering) has been shown to bind more strongly to Gb(3) than to Gb(4).

PubMed: 10745005
DOI: 10.1016/S0969-2126(00)00103-9

実験手法

X-RAY DIFFRACTION (2.35 Å)