1QOH
A MUTANT SHIGA-LIKE TOXIN IIE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-08-15 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 113.490, 54.510, 116.890 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 21.000 - 2.350 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BOV AND 2BOS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.100 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.360 | 2.490 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.084 | 0.330 |
| Total number of observations | 62524 * | |
| Number of reflections | 34188 | |
| <I/σ(I)> | 8.415 | 0.968 |
| Completeness [%] | 60.1 | 24.85 * |
| Redundancy | 1.83 | 1.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 8 (%) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | imidazole | 0.1 (M) |
