1QLN

STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX

Summary for 1QLN

• Entry DOI: 10.2210/pdb1qln/pdb
• Related: 1ARO 1CEZ 4RNP
• Descriptor: BACTERIOPHAGE T7 RNA POLYMERASE, DNA (5-D(P*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*TP*A)-3), RNA (5- R(PPP*GP*GP*G)-3), ... (5 entities in total)
• Functional Keywords: nucleotidyltransferase, t7 rna polymerase, protein/dna/rna
• Biological source: BACTERIOPHAGE T7; More
• Total number of polymer chains: 4
• Total formula weight: 112006.59

Authors

Cheetham, G.M.T.,Steitz, T.A. (deposition date: 1999-09-01, release date: 2000-02-04, Last modification date: 2023-12-13)

Primary citation

Cheetham, G.M.T.,Steitz, T.A.
Structure of a Transcribing T7 RNA Polymerase Initiation Complex
Science, 286:2305-, 1999

PubMed Abstract: The structure of a T7 RNA polymerase (T7 RNAP) initiation complex captured transcribing a trinucleotide of RNA from a 17-base pair promoter DNA containing a 5-nucleotide single-strand template extension was determined at a resolution of 2.4 angstroms. Binding of the upstream duplex portion of the promoter occurs in the same manner as that in the open promoter complex, but the single-stranded template is repositioned to place the +4 base at the catalytic active site. Thus, synthesis of RNA in the initiation phase leads to accumulation or "scrunching" of the template in the enclosed active site pocket of T7 RNAP. Only three base pairs of heteroduplex are formed before the RNA peels off the template.

PubMed: 10600732
DOI: 10.1126/SCIENCE.286.5448.2305

Experimental method

X-RAY DIFFRACTION (2.4 Å)