1QLN
STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | BRANDEIS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 221.200, 73.600, 80.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cez |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.078 | 0.370 |
Number of reflections | 51537 * | |
<I/σ(I)> | 10.6 | 1.6 |
Completeness [%] | 97.8 | 95.7 |
Redundancy | 3.8 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8.7 | pH 8.70 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | T7 RNAP | 0.3 (mM) | |
2 | 1 | 1 | T7 promoter | 0.35 (mM) | |
3 | 1 | 1 | ribonucleotides GTP | 4 (mM) | |
4 | 1 | 1 | uridine 5'-triphosphate | 4 (mM) | |
5 | 1 | 1 | cytidine 5'-triphosphate | 4 (mM) | |
6 | 1 | 1 | alpha, beta-methylene ATP | 4 (mM) | |
7 | 1 | 1 | 15 (mM) |