1QKL
hRPABC14.4, essential subunit of human RNA polymerases I, II and III
Summary for 1QKL
| Entry DOI | 10.2210/pdb1qkl/pdb |
| Descriptor | DNA-DIRECTED RNA POLYMERASE II 14.4 KD POLYPEPTIDE (1 entity in total) |
| Functional Keywords | rna polymerase, transcription |
| Biological source | HOMO SAPIENS (HUMAN) |
| Total number of polymer chains | 1 |
| Total formula weight | 14491.03 |
| Authors | Del Rio-Portilla, F.,Gaskell, A.G.,Gilbert, D.,Ladias, J.A.A.,Wagner, G. (deposition date: 1999-07-26, release date: 1999-11-07, Last modification date: 2024-05-15) |
| Primary citation | Del Rio-Portilla, F.,Gaskell, A.G.,Gilbert, D.,Ladias, J.A.A.,Wagner, G. Solution Structure of the Hrpabc14.4 Subunit of Human RNA Polymerases Nat.Struct.Biol., 6:1039-, 1999 Cited by PubMed Abstract: The protein hRPABC14.4 is an essential subunit of human RNA polymerases I, II, and III and is required for the transcription of all human nuclear genes. The structure of hRPABC14.4 was determined by nuclear magnetic resonance spectroscopy. The protein fold comprises a highly conserved central domain forming two antiparallel alpha-helices flanked by the less conserved N- and C-terminal regions forming a five-stranded beta-sandwich. Amino acids from the two helices participate in the generation of a hydrophobic surface area which is conserved in all eukaryotic and archaeal homologous subunits, and likely constitutes a critical macromolecular interaction interface. The hRPABC14.4 structure accounts for mutagenesis results in Saccharomyces cerevisiae and provides a structural working model for elucidating the role of this subunit in the molecular architecture and function of the human nuclear RNA polymerases. PubMed: 10542096DOI: 10.1038/14923 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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