1Q8K

Solution structure of alpha subunit of human eIF2

1Q8K の概要

• エントリーDOI: 10.2210/pdb1q8k/pdb
• NMR情報: BMRB: 5917
• 分子名称: Eukaryotic translation initiation factor 2 subunit 1 (1 entity in total)
• 機能のキーワード: translation, translation initiation, eukaryotic translation initiation factor 2
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasmic granule (By similarity): P05198
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35741.76

構造登録者

Ito, T.,Marintchev, A.,Wagner, G. (登録日: 2003-08-21, 公開日: 2004-09-07, 最終更新日: 2024-05-22)

主引用文献

Ito, T.,Marintchev, A.,Wagner, G.
Solution Structure of Human Initiation Factor eIF2alpha Reveals Homology to the Elongation Factor eEF1B.
STRUCTURE, 12:1693-1704, 2004

PubMed Abstract: The GTP-bound form of the trimeric eukaryotic translation initiation factor 2 (eIF2) transfers aminoacylated initiator methionyl tRNA onto the 40S ribosome. We have solved with solution NMR the structure of the alpha subunit of human eIF2 (heIF2alpha). The protein consists of two domains that are mobile relative to each other. The N-terminal domain has an S1-type oligonucleotide/oligosaccharide binding-fold subdomain and an alpha-helical subdomain. The C-terminal domain adopts an alphabeta-fold very similar to the C-terminal domain of elongation factor (eEF) 1Balpha, the guanine-nucleotide exchange factor for eEF1A. The structural and functional similarities found between eIF2alpha/eIF2gamma and eEF1Balpha/eEF1A suggest a model for the interaction of eIF2alpha with eIF2gamma, and eIF2 with Met-tRNAiMet. It further indicates a previously unrecognized evolutionary lineage of eIF2alpha/gamma from the functionally related elongation factor eEF1Balpha/eEF1A complex.

PubMed: 15341733
DOI: 10.1016/j.str.2004.07.010

実験手法

SOLUTION NMR