1Q5M

Binary complex of rabbit 20alpha-hydroxysteroid dehydrogenase with NADPH

Summary for 1Q5M

• Entry DOI: 10.2210/pdb1q5m/pdb
• Related: 1Q13
• Descriptor: Prostaglandin-E2 9-reductase, SULFATE ION, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• Functional Keywords: nadph, hsd, hydroxysteroid dehydrogenase, aldo-keto reductase, oxidoreductase
• Biological source: Oryctolagus cuniculus (rabbit)
• Cellular location: Cytoplasm: P80508
• Total number of polymer chains: 2
• Total formula weight: 74861.05

Authors

Couture, J.F.,Legrand, P.,Cantin, L.,Labrie, F.,Luu-The, V.,Breton, R. (deposition date: 2003-08-08, release date: 2004-05-18, Last modification date: 2024-02-14)

Primary citation

Couture, J.F.,Legrand, P.,Cantin, L.,Labrie, F.,Luu-The, V.,Breton, R.
Loop Relaxation, A Mechanism that Explains the Reduced Specificity of Rabbit 20alpha-Hydroxysteroid Dehydrogenase, A Member of the Aldo-Keto Reductase Superfamily.
J.Mol.Biol., 339:89-102, 2004

PubMed Abstract: The aldo-keto reductase rabbit 20alpha-hydroxysteroid dehydrogenase (rb20alpha-HSD; AKR1C5) is less selective than other HSDs, since it exerts its activity both on androgens (C19 steroids) and progestins (C21 steroids). In order to identify the molecular determinants responsible for this reduced selectivity, binary (NADPH) and ternary (NADP(+)/testosterone) complex structures were solved to 1.32A and 2.08A resolution, respectively. Inspection of the cofactor-binding cavity led to the identification of a new interaction between side-chains of residues His222 and Lys270, which cover the central phosphate chain of the cofactor, reminiscent of the "safety-belt" found in other aldo-keto reductases. Testosterone is stabilized by a phenol/benzene tunnel composed of side-chains of numerous residues, among which Phe54, which forces the steroid to take up an orientation markedly contrasting with that found in HSD ternary complexes reported. Combining structural, site-directed mutagenesis, kinetic and fluorescence titration studies, we found that the selectivity of rb20alpha-HSD is mediated by (i) the relaxation of loop B (residues 223-230), partly controlled by the nature of residue 230, (ii) the nature of the residue found at position 54, and (iii) the residues found in the C-terminal tail of the protein especially the side-chain of the amino acid 306.

PubMed: 15123423
DOI: 10.1016/j.jmb.2004.03.035

Experimental method

X-RAY DIFFRACTION (1.32 Å)