1Q5A

S-shaped trans interactions of cadherins model based on fitting C-cadherin (1L3W) to 3D map of desmosomes obtained by electron tomography

1Q5A の概要

• エントリーDOI: 10.2210/pdb1q5a/pdb
• 関連するPDBエントリー: 1L3W 1Q55 1Q5B 1Q5C
• EMDBエントリー: 1052
• 分子名称: EP-cadherin, 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-alpha-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: cadherin; trans interaction; desmosome; junction; adhesion, structural protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 203104.82

構造登録者

He, W.,Cowin, P.,Stokes, D.L. (登録日: 2003-08-06, 公開日: 2003-10-07, 最終更新日: 2020-07-29)

主引用文献

He, W.,Cowin, P.,Stokes, D.L.
Untangling Desmosomal Knots with Electron Tomography
Science, 302:109-113, 2003

PubMed Abstract: Cell adhesion by adherens junctions and desmosomes relies on interactions between cadherin molecules. However, the molecular interfaces that define molecular specificity and that mediate adhesion remain controversial. We used electron tomography of plastic sections from neonatal mouse skin to visualize the organization of desmosomes in situ. The resulting three-dimensional maps reveal individual cadherin molecules forming discrete groups and interacting through their tips. Fitting of an x-ray crystal structure for C-cadherin to these maps is consistent with a flexible intermolecular interface mediated by an exchange of amino-terminal tryptophans. This flexibility suggests a novel mechanism for generating both cis and trans interactions and for propagating these adhesive interactions along the junction.

PubMed: 14526082
DOI: 10.1126/science.1086957

実験手法

ELECTRON MICROSCOPY (30 Å)