1Q40

Crystal structure of the C. albicans Mtr2-Mex67 M domain complex

Summary for 1Q40

• Entry DOI: 10.2210/pdb1q40/pdb
• Related: 1OF5 1Q42
• Descriptor: MRNA TRANSPORT REGULATOR Mtr2, mRNA export factor MEX67, GLYCEROL, ... (4 entities in total)
• Functional Keywords: ntf2-fold; nuclear export, translation
• Biological source: Candida albicans; More
• Cellular location: Nucleus: P84148 P84148
• Total number of polymer chains: 4
• Total formula weight: 96108.03

Authors

Senay, C.,Ferrari, P.,Rocher, C.,Rieger, K.J.,Winter, J.,Platel, D.,Bourne, Y. (deposition date: 2003-08-01, release date: 2003-12-09, Last modification date: 2011-07-13)

Primary citation

Senay, C.,Ferrari, P.,Rocher, C.,Rieger, K.J.,Winter, J.,Platel, D.,Bourne, Y.
The mtr2-mex67 ntf2-like domain complex: Structural insights into a dual role of MTR2 for yeast nuclear export
J.Biol.Chem., 278:48395-48403, 2003

PubMed Abstract: The formation of the Mtr2-Mex67 heterodimer is essential for yeast mRNA export as it constitutes a key nuclear component for shuttling mRNA between the nuclear and cytoplasm compartments through the nuclear pore complex. We report the crystal structures of apo-Mtr2 from the human pathogen Candida albicans and of its complex with the Mex67 NTF2-like domain. Compared with other members of the NTF2 fold family, Mtr2 displays novel structural features involved in the nuclear export of the large ribosomal subunit and consistent with a dual functional role of Mtr2 during yeast nuclear export events. The structure of the Mtr2-Mex67 NTF2-like domain complex, which overall is similar to those of the human and Saccharomyces cerevisiae homologs, unveils three putative Phe-Gly repeat binding sites, of which one contributes to the heterodimer interface. These structures exemplify an unrecognized adaptability of the NTF2 building block in evolution, identify novel structural determinants associated with key biological functions at the molecular surface of the yeast Mtr2-Mex67 complex, and suggest that the yeast and human mRNA export machineries may differ.

PubMed: 14504280
DOI: 10.1074/jbc.M308275200

Experimental method

X-RAY DIFFRACTION (1.95 Å)