1Q3I

Crystal Structure of Na,K-ATPase N-domain

Summary for 1Q3I

Entry DOI	10.2210/pdb1q3i/pdb
Descriptor	Na,K-ATPase, NICKEL (II) ION (3 entities in total)
Functional Keywords	anti-parallel beta sheet, hydrolase
Biological source	Sus scrofa (pig)
Total number of polymer chains	1
Total formula weight	24304.45
Authors	Hakansson, K.O. (deposition date: 2003-07-30, release date: 2004-08-03, Last modification date: 2024-02-14)
Primary citation	Hakansson, K.O. The Crystallographic Structure of Na,K-ATPase N-domain at 2.6 A Resolution J.Mol.Biol., 332:1175-1182, 2003 Cited by PubMed Abstract: The structure of the N-domain of porcine alpha(2) Na,K-ATPase was determined crystallographically to 3.2A resolution by isomorphous heavy-atom replacement using a single mercury derivative. The structure was finally refined against 2.6A resolution synchrotron data. The domain forms a seven-stranded antiparallel beta-sheet with two additional beta-strands forming a hairpin and five alpha-helices. Approximately 75% of the residues were superimposable with residues from the structure of Ca-ATPase N-domain, and a structure-based sequence alignment is presented. The positions of key residues are discussed in relation to the pattern of hydrophobicity, charge and sequence conservation of the molecular surface. The structure of a hexahistidine tag binding to nickel ions is presented. PubMed: 14499619 DOI: 10.1016/j.jmb.2003.07.012 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation