1PZB

THE CRYSTAL STRUCTURES OF REDUCED PSEUDOAZURIN FROM ALCALIGENES FAECALIS S-6 AT TWO PH VALUES

1PZB の概要

• エントリーDOI: 10.2210/pdb1pzb/pdb
• 関連するPDBエントリー: 1PAZ 1PZA
• 分子名称: PSEUDOAZURIN, COPPER (II) ION (3 entities in total)
• 機能のキーワード: electron transfer(cuproprotein)
• 由来する生物種: Alcaligenes faecalis
• 細胞内の位置: Periplasm: P04377
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13447.06

構造登録者

Petratos, K. (登録日: 1994-08-03, 公開日: 1994-11-30, 最終更新日: 2024-02-14)

主引用文献

Vakoufari, E.,Wilson, K.S.,Petratos, K.
The crystal structures of reduced pseudoazurin from Alcaligenes faecalis S-6 at two pH values.
FEBS Lett., 347:203-206, 1994

PubMed Abstract: The structures of the reduced (Cu1+) blue-copper protein pseudoazurin from Alcaligenes faecalis strain S-6 are refined at pH 7.8 and 4.4 using X-ray diffraction data to 1.8 A resolution. The final R-factors for the high and low pH structures are 0.178 and 0.177, respectively. Comparing the reduced pseudoazurin at pH 7.8 with the oxidised (Cu2+) molecule, small changes are observed in the vicinity of the copper site and on the protein surface. At pH 4.4 the copper substituent imidazole of His81 rotates away from the metal with a concurrent movement of the latter towards the plane of the remaining three ligands (S gamma-Cys78, N delta 1-His40 and S delta-Met86) thus the geometry of the copper site becomes planar trigonal.

PubMed: 8034003
DOI: 10.1016/0014-5793(94)00544-3

実験手法

X-RAY DIFFRACTION (1.8 Å)