1PXW

Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii

1PXW の概要

• エントリーDOI: 10.2210/pdb1pxw/pdb
• 分子名称: LSU ribosomal protein L7AE (2 entities in total)
• 機能のキーワード: ribosome
• 由来する生物種: Pyrococcus abyssi
• 細胞内の位置: Cytoplasm : P62008
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28154.89

構造登録者

Charron, C.,Manival, X.,Charpentier, B.,Branlant, C.,Aubry, A. (登録日: 2003-07-07, 公開日: 2004-01-27, 最終更新日: 2024-02-14)

主引用文献

Charron, C.,Manival, X.,Charpentier, B.,Branlant, C.,Aubry, A.
Purification, crystallization and preliminary X-ray diffraction data of L7Ae sRNP core protein from Pyrococcus abyssii.
Acta Crystallogr.,Sect.D, 60:122-124, 2004

PubMed Abstract: The L7Ae sRNP core protein from Pyrococcus abyssii was crystallized using the sitting-drop vapour-diffusion method. Crystals were obtained in the presence of MgCl(2), PEG 2000 MME and acetate buffer at pH 4.0. A native data set has been collected at 2.9 A resolution using a rotating-anode generator at room temperature. Crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 70.7, b = 112.9, c = 34.8 A. There are two monomers of MW 14 200 Da per asymmetric unit and the packing density V(M) is 2.45 A(3) Da(-1). A molecular-replacement analysis gave solutions for the rotation and translation functions.

PubMed: 14684904
DOI: 10.1107/S090744490302239X

実験手法

X-RAY DIFFRACTION (1.94 Å)