1PRM

TWO BINDING ORIENTATIONS FOR PEPTIDES TO SRC SH3 DOMAIN: DEVELOPMENT OF A GENERAL MODEL FOR SH3-LIGAND INTERACTIONS

1PRM の概要

• エントリーDOI: 10.2210/pdb1prm/pdb
• 分子名称: C-SRC TYROSINE KINASE SH3 DOMAIN, PROLINE-RICH LIGAND PLR1 (AFAPPLPRR) (2 entities in total)
• 機能のキーワード: complex (signal transduction-peptide), complex (signal transduction-peptide) complex, complex (signal transduction/peptide)
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Cell membrane (By similarity): P00523
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8029.88

構造登録者

Feng, S.,Chen, J.K.,Yu, H.,Simon, J.A.,Schreiber, S.L. (登録日: 1994-10-10, 公開日: 1995-02-07, 最終更新日: 2024-05-22)

主引用文献

Feng, S.,Chen, J.K.,Yu, H.,Simon, J.A.,Schreiber, S.L.
Two binding orientations for peptides to the Src SH3 domain: development of a general model for SH3-ligand interactions.
Science, 266:1241-1247, 1994

PubMed Abstract: Solution structures of two Src homology 3 (SH3) domain-ligand complexes have been determined by nuclear magnetic resonance. Each complex consists of the SH3 domain and a nine-residue proline-rich peptide selected from a large library of ligands prepared by combinatorial synthesis. The bound ligands adopt a left-handed polyproline type II (PPII) helix, although the amino to carboxyl directionalities of their helices are opposite. The peptide orientation is determined by a salt bridge formed by the terminal arginine residues of the ligands and the conserved aspartate-99 of the SH3 domain. Residues at positions 3, 4, 6, and 7 of both peptides also intercalate into the ligand-binding site; however, the respective proline and nonproline residues show exchanged binding positions in the two complexes. These structural results led to a model for the interactions of SH3 domains with proline-rich peptides that can be used to predict critical residues in complexes of unknown structure. The model was used to identify correctly both the binding orientation and the contact and noncontact residues of a peptide derived from the nucleotide exchange factor Sos in association with the amino-terminal SH3 domain of the adaptor protein Grb2.

PubMed: 7526465

実験手法

SOLUTION NMR