1PP5

Structure of Antibacterial Peptide Microcin J25: a 21-Residue Lariat Protoknot

Summary for 1PP5

• Entry DOI: 10.2210/pdb1pp5/pdb
• NMR Information: BMRB: 5859
• Related PRD ID: PRD_000184
• Descriptor: microcin J25 (1 entity in total)
• Functional Keywords: lariat, protoknot, backbone-sidechain amide linkage, structural genomics, psi, protein structure initiative, northeast structural genomics consortium, nesg, antibiotic
• Biological source: Escherichia coli
• Cellular location: Secreted: Q9X2V7
• Total number of polymer chains: 1
• Total formula weight: 2126.35

Authors

Bayro, M.J.,Swapna, G.V.T.,Huang, J.Y.,Ma, L.-C.,Mukhopadhyay, J.,Ebright, R.H.,Montelione, G.T.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2003-06-16, release date: 2003-10-28, Last modification date: 2012-12-12)

Primary citation

Bayro, M.J.,Mukhopadhyay, J.,Swapna, G.V.T.,Huang, J.Y.,Ma, L.-C.,Sineva, E.,Dawson, P.E.,Montelione, G.T.,Ebright, R.H.
Structure of Antibacterial Peptide Microcin J25: A 21-Residue Lariat Protoknot.
J.Am.Chem.Soc., 125:12382-12383, 2003

PubMed Abstract: The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue "lariat protoknot", consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.

PubMed: 14531661
DOI: 10.1021/ja036677e

Experimental method

SOLUTION NMR