1POB

CRYSTAL STRUCTURE OF COBRA-VENOM PHOSPHOLIPASE A2 IN A COMPLEX WITH A TRANSITION-STATE ANALOGUE

Summary for 1POB

• Entry DOI: 10.2210/pdb1pob/pdb
• Descriptor: PHOSPHOLIPASE A2, CALCIUM ION, 1-O-OCTYL-2-HEPTYLPHOSPHONYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE, ... (4 entities in total)
• Functional Keywords: hydrolase
• Biological source: Naja atra (Chinese cobra)
• Total number of polymer chains: 2
• Total formula weight: 27448.64

Authors

White, S.P.,Scott, D.L.,Otwinowski, Z.,Sigler, P.B. (deposition date: 1992-09-07, release date: 1993-10-31, Last modification date: 2019-08-14)

Primary citation

White, S.P.,Scott, D.L.,Otwinowski, Z.,Gelb, M.H.,Sigler, P.B.
Crystal structure of cobra-venom phospholipase A2 in a complex with a transition-state analogue.
Science, 250:1560-1563, 1990

PubMed Abstract: The crystal structure of a complex between a phosphonate transition-state analogue and the phospholipase A2 (PLA2) from Naja naja atra venom has been solved and refined to a resolution of 2.0 angstroms. The identical stereochemistry of the two complexes that comprise the crystal's asymmetric unit indicates both the manner in which the transition state is stabilized and how the hydrophobic fatty acyl chains of the substrate are accommodated by the enzyme during interfacial catalysis. The critical features that suggest the chemistry of binding and catalysis are the same as those seen in the crystal structure of a similar complex formed with the evolutionarily distant bee-venom PLA2.

PubMed: 2274787

Experimental method

X-RAY DIFFRACTION (2 Å)