1PMY

REFINED CRYSTAL STRUCTURE OF PSEUDOAZURIN FROM METHYLOBACTERIUM EXTORQUENS AM1 AT 1.5 ANGSTROMS RESOLUTION

Summary for 1PMY

• Entry DOI: 10.2210/pdb1pmy/pdb
• Descriptor: PSEUDOAZURIN, COPPER (II) ION (3 entities in total)
• Functional Keywords: electron transfer(cuproprotein)
• Biological source: Methylobacterium extorquens
• Total number of polymer chains: 1
• Total formula weight: 13474.14

Authors

Inoue, T.,Kai, Y.,Harada, S.,Kasai, N.,Ohshiro, Y.,Suzuki, S.,Kohzuma, T.,Tobari, J. (deposition date: 1994-01-28, release date: 1994-07-31, Last modification date: 2024-02-14)

Primary citation

Inoue, T.,Kai, Y.,Harada, S.,Kasai, N.,Ohshiro, Y.,Suzuki, S.,Kohzuma, T.,Tobari, J.
Refined crystal structure of pseudoazurin from Methylobacterium extorquens AM1 at 1.5 A resolution.
Acta Crystallogr.,Sect.D, 50:317-328, 1994

PubMed Abstract: The crystal structure of pseudoazurin from Methylobacterium extorquens AM1 (PAZAM1) has been solved by the molecular replacement method using copper-copper distances as translation parameters, which were obtained from difference Patterson maps calculated with the synchrotron radiation data containing the multiwavelength anomalous-dispersion effect. The structure refinement was carried out by the use of molecular dynamics optimization and the restrained least-squares method. The final crystallographic R factor was 19.9% for the 14 365 reflections greater than 3sigma between 1.5 and 8.0 A resolution. This report describes the characteristic features of the structure of PAZAM 1 as well as the effectiveness of synchrotron radiation for structure analysis of metalloproteins. The environment of the metal active site and the structural differences among blue-copper proteins are discussed.

PubMed: 15299445
DOI: 10.1107/S0907444994000260

Experimental method

X-RAY DIFFRACTION (1.5 Å)