1PLW

NMR structure of Methionine-Enkephalin in fast tumbling DMPC/DHPC bicelles

Summary for 1PLW

• Entry DOI: 10.2210/pdb1plw/pdb
• Related: 1PLX
• NMR Information: BMRB: 5915
• Descriptor: Met-enkephalin 1 (1 entity in total)
• Functional Keywords: neuropeptide
• Cellular location: Secreted: P01210
• Total number of polymer chains: 1
• Total formula weight: 573.66

Authors

Marcotte, I.,Separovic, F.,Auger, M.,Gagne, S.M. (deposition date: 2003-06-09, release date: 2004-03-16, Last modification date: 2024-05-22)

Primary citation

Marcotte, I.,Separovic, F.,Auger, M.,Gagne, S.M.
A multidimensional (1)h NMR investigation of the conformation of methionine-enkephalin in fast-tumbling bicelles.
Biophys.J., 86:1587-1600, 2004

PubMed Abstract: Enkephalins are pentapeptides found in the central nervous system. It is believed that these neuropeptides interact with the nerve cell membrane to adopt a conformation suitable for their binding to an opiate receptor. In this work, we have determined the three-dimensional structure of methionine-enkephalin (Menk) in fast-tumbling bicelles using multidimensional (1)H NMR. Bicelles were selected as model membranes because both their bilayer organization and composition resemble those of natural biomembranes. The effect of the membrane composition on the peptide conformation was explored using both zwitterionic (PC bicelles) and negatively charged bicelles (Bic/PG). Pulsed field gradient experiments allowed the determination of the proportion of Menk bound to the model membranes. Approximately 60% of the water-soluble enkephalin was found to associate to the bicellar systems. Structure calculations from torsion angle and NOE-based distance constraints suggest the presence of both micro - and delta-selective conformers of Menk in each system and slightly different conformers in PC bicelles and Bic/PG. As opposed to previous studies of enkephalins in membrane mimetic systems, our results show that these opiate peptides could adopt several conformations in a membrane environment, which is consistent with the flexibility and poor selectivity of enkephalins.

PubMed: 14990485

Experimental method

SOLUTION NMR