1PID

BOVINE DESPENTAPEPTIDE INSULIN

1PID の概要

• エントリーDOI: 10.2210/pdb1pid/pdb
• 分子名称: DESPENTAPEPTIDE INSULIN (3 entities in total)
• 機能のキーワード: hormone, glucose metabolism
• 由来する生物種: Bos taurus (cattle); 詳細
• 細胞内の位置: Secreted: P01317 P01317
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 10363.84

構造登録者

Holden, P.H.,Papiz, M.,Dodson, G.G. (登録日: 1995-11-22, 公開日: 1996-12-07, 最終更新日: 2024-10-23)

主引用文献

Brange, J.,Dodson, G.G.,Edwards, D.J.,Holden, P.H.,Whittingham, J.L.
A model of insulin fibrils derived from the x-ray crystal structure of a monomeric insulin (despentapeptide insulin).
Proteins, 27:507-516, 1997

PubMed Abstract: The crystal structure of despentapeptide insulin, a monomeric insulin, has been refined at 1.3 A spacing and subsequently used to predict and model the organization in the insulin fibril. The model makes use of the contacts in the densely packed despentapeptide insulin crystal, and takes into account other experimental evidence, including binding studies with Congo red. The dimensions of this model fibril correspond well with those measured experimentally, and the monomer-monomer contacts within the fibril are in accordance with the known physical chemistry of insulin fibrils. Using this model, it may be possible to predict mutations in insulin that might alleviate problems associated with fibril formation during insulin therapy.

PubMed: 9141131
DOI: 10.1002/(SICI)1097-0134(199704)27:4<507::AID-PROT4>3.3.CO;2-H

実験手法

X-RAY DIFFRACTION (1.3 Å)