1PEY
Crystal structure of the Response Regulator Spo0F complexed with Mn2+
Summary for 1PEY
| Entry DOI | 10.2210/pdb1pey/pdb |
| Related | 1NAT 1SRR |
| Descriptor | Sporulation initiation phosphotransferase F, MANGANESE (II) ION (3 entities in total) |
| Functional Keywords | cation binding, (alpha/beta) protein, transferase |
| Biological source | Bacillus subtilis |
| Total number of polymer chains | 3 |
| Total formula weight | 42670.51 |
| Authors | Mukhopadhyay, D.,Sen, U.,Zapf, J.,Varughese, K.I. (deposition date: 2003-05-22, release date: 2004-05-18, Last modification date: 2024-02-14) |
| Primary citation | Mukhopadhyay, D.,Sen, U.,Zapf, J.,Varughese, K.I. Metals in the sporulation phosphorelay: manganese binding by the response regulator Spo0F. Acta Crystallogr.,Sect.D, 60:638-645, 2004 Cited by PubMed Abstract: As a part of studies on the structural characterization of the components of the sporulation phosphorelay in Bacillus subtilis, the crystal structure of the manganese derivative of an intermediate signal transducer, Spo0F, has been elucidated at 2.25 A resolution. The calcium complex and the apo structures have been analyzed previously. In apo Spo0F, the active-site cation cavity is only partially formed and it only becomes completed upon metal coordination. The carbonyl of Lys56 is coordinated to the metal and interestingly the side chain of Lys56 exists in a variety of conformations in the three crystal structures of Spo0F. The affinity of the magnesium ion for Spo0F is in fact low; however, it binds Spo0F when it is in complex with Spo0B. It is proposed that the existence of a deep pocket which extends from the surface to the metal site could attract and direct the metal, thereby facilitating the metal binding of the complex. PubMed: 15039551DOI: 10.1107/S0907444904002148 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.25 Å) |
Structure validation
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