1PDQ

Polycomb chromodomain complexed with the histone H3 tail containing trimethyllysine 27.

1PDQ の概要

• エントリーDOI: 10.2210/pdb1pdq/pdb
• 関連するPDBエントリー: 1guw 1kna 1kne
• 分子名称: Polycomb protein, Histone H3.3 (3 entities in total)
• 機能のキーワード: methyllysine, chromodomain, histone h3, polycomb, lysine methylation, trimethyllysine, cation-pi, chromo, structural protein
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• 細胞内の位置: Nucleus (By similarity): P26017; Nucleus: P06351
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 10782.35

構造登録者

Fischle, W.,Wang, Y.,Jacobs, S.A.,Kim, Y.,Allis, C.D.,Khorasanizadeh, S. (登録日: 2003-05-20, 公開日: 2003-08-26, 最終更新日: 2023-08-16)

主引用文献

Fischle, W.,Wang, Y.,Jacobs, S.A.,Kim, Y.,Allis, C.D.,Khorasanizadeh, S.
Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains
Genes Dev., 17:1870-1881, 2003

PubMed Abstract: On the histone H3 tail, Lys 9 and Lys 27 are both methylation sites associated with epigenetic repression, and reside within a highly related sequence motif ARKS. Here we show that the chromodomain proteins Polycomb (Pc) and HP1 (heterochromatin protein 1) are highly discriminatory for binding to these sites in vivo and in vitro. In Drosophila S2 cells, and on polytene chromosomes, methyl-Lys 27 and Pc are both excluded from areas that are enriched in methyl-Lys 9 and HP1. Swapping of the chromodomain regions of Pc and HP1 is sufficient for switching the nuclear localization patterns of these factors, indicating a role for their chromodomains in both target site binding and discrimination. To better understand the molecular basis for the selection of methyl-lysine binding sites, we solved the 1.8 A structure of the Pc chromodomain in complex with a H3 peptide bearing trimethyl-Lys 27, and compared it with our previously determined structure of the HP1 chromodomain in complex with a H3 peptide bearing trimethyl-Lys 9. The Pc chromodomain distinguishes its methylation target on the H3 tail via an extended recognition groove that binds five additional residues preceding the ARKS motif.

PubMed: 12897054
DOI: 10.1101/gad.1110503

実験手法

X-RAY DIFFRACTION (1.76 Å)