1PDL

Fitting of gp5 in the cryoEM reconstruction of the bacteriophage T4 baseplate

1PDL の概要

• エントリーDOI: 10.2210/pdb1pdl/pdb
• 関連するPDBエントリー: 1K28
• EMDBエントリー: 1048
• 分子名称: Tail-associated lysozyme (1 entity in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 189551.17

構造登録者

Kostyuchenko, V.A.,Leiman, P.G.,Chipman, P.R.,Kanamaru, S.,van Raaij, M.J.,Arisaka, F.,Mesyanzhinov, V.V.,Rossmann, M.G. (登録日: 2003-05-19, 公開日: 2003-09-09, 最終更新日: 2024-02-14)

主引用文献

Kostyuchenko, V.A.,Leiman, P.G.,Chipman, P.R.,Kanamaru, S.,van Raaij, M.J.,Arisaka, F.,Mesyanzhinov, V.V.,Rossmann, M.G.
Three-dimensional structure of the bacteriophage T4 baseplate
Nat.Struct.Biol., 10:688-693, 2003

PubMed Abstract: The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.

PubMed: 12923574
DOI: 10.1038/nsb970

実験手法

ELECTRON MICROSCOPY (12 Å)