1P6T

Structure characterization of the water soluble region of P-type ATPase CopA from Bacillus subtilis

Summary for 1P6T

• Entry DOI: 10.2210/pdb1p6t/pdb
• Related: 1KQK 1OQ3
• NMR Information: BMRB: 5813
• Descriptor: Potential copper-transporting ATPase (1 entity in total)
• Functional Keywords: copa; p-type atpase; water-soluble region; beta-alpha-beta-beta-alpha-beta fold; nmr, structural proteomics in europe, spine, structural genomics, hydrolase
• Biological source: Bacillus subtilis
• Cellular location: Cell membrane; Multi-pass membrane protein: O32220
• Total number of polymer chains: 1
• Total formula weight: 16400.75

Authors

Banci, L.,Bertini, I.,Ciofi-Baffoni, S.,Gonnelli, L.,Su, X.C.,Structural Proteomics in Europe (SPINE) (deposition date: 2003-04-30, release date: 2003-12-16, Last modification date: 2024-05-22)

Primary citation

Banci, L.,Bertini, I.,Ciofi-Baffoni, S.,Gonnelli, L.,Su, X.C.
Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis.
J.Biol.Chem., 278:50506-50513, 2003

PubMed Abstract: The solution structure of the N-terminal region (151 amino acids) of a copper ATPase, CopA, from Bacillus subtilis, is reported here. It consists of two domains, CopAa and CopAb, linked by two amino acids. It is found that the two domains, which had already been separately characterized, interact one to the other through a hydrogen bond network and a few hydrophobic interactions, forming a single rigid body. The two metal binding sites are far from one another, and the short link between the domains prevents them from interacting. This and the surface electrostatic potential suggest that each domain receives copper from the copper chaperone, CopZ, independently and transfers it to the membrane binding site of CopA. The affinity constants of silver(I) and copper(I) are similar for the two sites as monitored by NMR. Because the present construct "domain-short link-domain" is shared also by the last two domains of the eukaryotic copper ATPases and several residues at the interface between the two domains are conserved, the conclusions of the present study have general validity for the understanding of the function of copper ATPases.

PubMed: 14514665
DOI: 10.1074/jbc.M307389200

Experimental method

SOLUTION NMR