1P2V

H-RAS 166 in 60 % 1,6 hexanediol

1P2V の概要

• エントリーDOI: 10.2210/pdb1p2v/pdb
• 関連するPDBエントリー: 1P2S 1P2T 1P2U
• 分子名称: Transforming protein p21/H-RAS-1, MAGNESIUM ION, HEXANE-1,6-DIOL, ... (5 entities in total)
• 機能のキーワード: molecular switch protein, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Lipid-anchor; Cytoplasmic side: P01112
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19539.87

構造登録者

Buhrman, G.K.,de Serrano, V.,Mattos, C. (登録日: 2003-04-16, 公開日: 2003-08-05, 最終更新日: 2023-08-16)

主引用文献

Buhrman, G.K.,de Serrano, V.,Mattos, C.
Organic solvents order the dynamic switch II in Ras crystals
Structure, 11:747-751, 2003

PubMed Abstract: Room temperature crystal structures of crosslinked H-Ras bound to GMPPNP were solved in 50% 2,2,2-trifluoroethanol, 60% 1,6-hexanediol, and 50% isopropanol. The disordered switch II region of Ras is ordered in the presence of 2,2,2-trifluoroethanol or 1,6-hexanediol. The overall backbone conformation of switch II in these organic solvents is the same as in the Ras-GMPPNP complexes with RalGDS, PI(3) kinase, and RasGAP, indicating a biologically relevant form. Key polar interactions that stabilize the ordered switch are enhanced in the presence of hydrophobic cosolvents. These results suggest that hydrophobic solvents can be used in general to order short biologically relevant segments of disordered regions in protein crystals by favoring H-bonding interactions between atoms that are highly solvated and mobile in aqueous solution.

PubMed: 12842038
DOI: 10.1016/S0969-2126(03)00128-X

実験手法

X-RAY DIFFRACTION (2.3 Å)