1P2V

H-RAS 166 in 60 % 1,6 hexanediol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 32 2 1
Unit cell lengths	40.360, 40.360, 161.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.580 - 2.300
R-factor	0.21
Rwork	0.210
R-free	0.25000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	121p
RMSD bond length	0.006 *
RMSD bond angle	1.150 *
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.320
High resolution limit [Å]	2.300 *
Number of reflections	10944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		Scherer, A., (1989) J.Mol.Biol., 206, 257. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3-4 (mg/ml)
2	1	1	Tris-HCl	64 (mM)
3	1	1	dithioerithritol	1 (mM)
4	1	1		10 (mM)
5	1	1	sodium azide	1 (mM)
6	1	1	PEG1450	20 (%(w/v))