1OUW

Crystal structure of Calystegia sepium agglutinin

1OUW の概要

• エントリーDOI: 10.2210/pdb1ouw/pdb
• 関連するPDBエントリー: 1C3K 1C3M 1C3N
• 分子名称: lectin, 1,2-ETHANEDIOL, IMIDAZOLE, ... (5 entities in total)
• 機能のキーワード: beta-prism fold, agglutinin, lectin, mannose-binding, jacalin-related, sugar binding protein
• 由来する生物種: Calystegia sepium (hedge bindweed)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 65045.73

構造登録者

Bourne, Y.,Roig-Zamboni, V.,Barre, A.,Peumans, W.J.,Astoul, C.H.,van Damme, E.J.M.,Rouge, P. (登録日: 2003-03-25, 公開日: 2003-11-11, 最終更新日: 2025-03-26)

主引用文献

Bourne, Y.,Roig-Zamboni, V.,Barre, A.,Peumans, W.J.,Astoul, C.H.,Van Damme, E.J.M.,Rouge, P.
The crystal structure of the Calystegia sepium agglutinin reveals a novel quaternary arrangement of lectin subunits with a beta-prism fold
J.Biol.Chem., 279:527-533, 2004

PubMed Abstract: The high number of quaternary structures observed for lectins highlights the important role of these oligomeric assemblies during carbohydrate recognition events. Although a large diversity in the mode of association of lectin subunits is frequently observed, the oligomeric assemblies of plant lectins display small variations within a single family. The crystal structure of the mannose-binding jacalin-related lectin from Calystegia sepium (Calsepa) has been determined at 1.37-A resolution. Calsepa exhibits the same beta-prism fold as identified previously for other members of the family, but the shape and the hydrophobic character of its carbohydrate-binding site is unlike that of other members, consistent with surface plasmon resonance analysis showing a preference for methylated sugars. Calsepa reveals a novel dimeric assembly markedly dissimilar to those described earlier for Heltuba and jacalin but mimics the canonical 12-stranded beta-sandwich dimer found in legume lectins. The present structure exemplifies the adaptability of the beta-prism building block in the evolution of plant lectins and highlights the biological role of these quaternary structures for carbohydrate recognition.

PubMed: 14561768
DOI: 10.1074/jbc.M308218200

実験手法

X-RAY DIFFRACTION (1.37 Å)