1ORQ

X-ray structure of a voltage-dependent potassium channel in complex with an Fab

1ORQ の概要

• エントリーDOI: 10.2210/pdb1orq/pdb
• 関連するPDBエントリー: 1ORS
• 分子名称: 6E1 Fab light chain, 6E1 Fab heavy chain, potassium channel, ... (6 entities in total)
• 機能のキーワード: potassium channel, voltage-dependent, kvap, fab complex, membrane protein
• 由来する生物種: Aeropyrum pernix; 詳細
• 細胞内の位置: Cell membrane; Single-pass membrane protein (Potential): P01865; Cell membrane; Multi-pass membrane protein: Q9YDF8
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 72602.72

構造登録者

Jiang, Y.,Lee, A.,Chen, J.,Ruta, V.,Cadene, M.,Chait, B.T.,MacKinnon, R. (登録日: 2003-03-14, 公開日: 2003-05-06, 最終更新日: 2024-11-20)

主引用文献

Jiang, Y.,Lee, A.,Chen, J.,Ruta, V.,Cadene, M.,Chait, B.T.,MacKinnon, R.
X-ray structure of a voltage-dependent K+ channel
Nature, 423:33-41, 2003

PubMed Abstract: Voltage-dependent K+ channels are members of the family of voltage-dependent cation (K+, Na+ and Ca2+) channels that open and allow ion conduction in response to changes in cell membrane voltage. This form of gating underlies the generation of nerve and muscle action potentials, among other processes. Here we present the structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix. We have determined a crystal structure of the full-length channel at a resolution of 3.2 A, and of the isolated voltage-sensor domain at 1.9 A, both in complex with monoclonal Fab fragments. The channel contains a central ion-conduction pore surrounded by voltage sensors, which form what we call 'voltage-sensor paddles'-hydrophobic, cationic, helix-turn-helix structures on the channel's outer perimeter. Flexible hinges suggest that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane.

PubMed: 12721618
DOI: 10.1038/nature01580

実験手法

X-RAY DIFFRACTION (3.2 Å)