1OPO

THE STRUCTURE OF CARNATION MOTTLE VIRUS

Summary for 1OPO

• Entry DOI: 10.2210/pdb1opo/pdb
• Related: 2TBV
• Descriptor: Coat protein, SULFATE ION, CALCIUM ION (3 entities in total)
• Functional Keywords: plant virus, carmovirus, virus/viral protein, tomato bushy stunt virus, icosahedral virus, virus
• Biological source: Carnation mottle virus
• Cellular location: Virion (By similarity): P04383
• Total number of polymer chains: 3
• Total formula weight: 113818.92

Authors

Morgunova, E.,Dauter, Z.,Fry, E.,Stuart, D.,Stel'mashchuk, V.,Mikhailov, A.M.,Wilson, K.S.,Vainshtein, B.K. (deposition date: 2003-03-06, release date: 2003-04-01, Last modification date: 2023-08-16)

Primary citation

Morgunova, E.,Dauter, Z.,Fry, E.,Stuart, D.,Stel'mashchuk, V.,Mikhailov, A.M.,Wilson, K.S.,Vainshtein, B.K.
The atomic structure of Carnation Mottle Virus capsid protein
Febs Lett., 338:267-271, 1994

PubMed Abstract: The structure of the Carnation Mottle Virus (CMtV) capsid protein has been determined at 3.2 A resolution by the method of molecular replacement. Three-dimensional data were collected from a small number of crystals (sp.g. I23, a = 382.6 A) using the synchrotron radiation with an image plate as detector. The coordinates of Tomato Bushy Stunt Virus (TBSV) were used as a searching model. Refinement of the coordinates of 7,479 non-hydrogen atoms performed by the program XPLOR, has led to an R-factor of 18.3%. It was found that the amino acid chain fold of capsid protein is very similar to that in other icosahedral viruses. However, there are some differences in the contact regions between protein subunits and also the lack of the beta-annulus around the 3-fold icosahedral axes. The structural and biochemical results lead us to consider an alternative assembly pathway.

PubMed: 8307192
DOI: 10.1016/0014-5793(94)80281-5

Experimental method

X-RAY DIFFRACTION (3.2 Å)