1OMY
Crystal Structure of a Recombinant alpha-insect Toxin BmKaIT1 from the scorpion Buthus martensii Karsch
Summary for 1OMY
| Entry DOI | 10.2210/pdb1omy/pdb |
| Descriptor | Alpha-neurotoxin TX12, CHLORIDE ION, ACETIC ACID, ... (4 entities in total) |
| Functional Keywords | alpha-insect toxin, sodium channel, toxin |
| Biological source | Mesobuthus martensii (Chinese scorpion) |
| Cellular location | Secreted: Q9GQW3 |
| Total number of polymer chains | 1 |
| Total formula weight | 7425.92 |
| Authors | Huang, Y.,Huang, Q.,Chen, H.,Tang, Y.,Miyake, H.,Kusunoki, M. (deposition date: 2003-02-26, release date: 2003-09-09, Last modification date: 2024-10-30) |
| Primary citation | Huang, Y.,Huang, Q.,Chen, H.,Tang, Y.,Miyake, H.,Kusunoki, M. Crystallization and preliminary crystallographic study of rBmKalphaIT1, a recombinant alpha-insect toxin from the scorpion Buthus martensii Karsch. Acta Crystallogr.,Sect.D, 59:1635-1636, 2003 Cited by PubMed Abstract: Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins for which no crystal structures are yet available. A novel alpha-insect toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1) was crystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKalphaIT1 molecule in the asymmetric unit. PubMed: 12925796DOI: 10.1107/S090744490301415X PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
Download full validation report
