1OJL

Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding

1OJL の概要

• エントリーDOI: 10.2210/pdb1ojl/pdb
• 分子名称: TRANSCRIPTIONAL REGULATORY PROTEIN ZRAR, PHOSPHATE ION, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: response regulator, two component system, aaa domain, ntrc family, dna-binding, transcription regulation
• 由来する生物種: SALMONELLA TYPHIMURIUM
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 203413.62

構造登録者

Sallai, L.,Tucker, P.A. (登録日: 2003-07-10, 公開日: 2005-05-19, 最終更新日: 2024-05-08)

主引用文献

Sallai, L.,Tucker, P.A.
Crystal structure of the central and C-terminal domain of the sigma(54)-activator ZraR.
J. Struct. Biol., 151:160-170, 2005

PubMed Abstract: The sigma(54)-dependent transcription in bacteria is associated with various stress and growth conditions. Activators of the sigma(54) protein contain a central domain belonging to the AAA+ superfamily of ATPases, members of which function in diverse cellular processes in both prokaryotic and eukaryotic cells. We describe the X-ray structure of an N-terminal domain deletion of the ZraR protein from Salmonella typhimurium, which is a homologue of the general nitrogen regulatory protein NtrC, at 3A resolution. The structure reveals a hexameric ring that is typical for AAA+ containing proteins but which differs from the heptameric ring found in the crystal structure of the AAA+ domain of NtrC1 from Aquifex aeolicus. The dimerisation interface between DNA-binding domains observed in the crystal structure suggests that dodecamers, rather than hexamers, might be the functionally important oligomer.

PubMed: 16005641
DOI: 10.1016/j.jsb.2005.05.006

実験手法

X-RAY DIFFRACTION (3 Å)