1OGV

Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides

Summary for 1OGV

• Entry DOI: 10.2210/pdb1ogv/pdb
• Related: 1AIG 1AIJ 1DS8 1DV3 1DV6 1E14 1E6D 1F6N 1FNP 1FNQ 1JGW 1JGX 1JGY 1JGZ 1JH0 1K6L 1K6N 1KBY 1L9B 1L9J 1M3X 1MPS 1PCR 1PSS 1PST 1QOV 1YST 2RCR 4RCR
• Descriptor: Reaction center protein H chain, Reaction center protein L chain, Reaction center protein M chain, ... (10 entities in total)
• Functional Keywords: reaction centre, photosynthesis, charge separation, integral membrane protein, lipid, electron transport, bacteriochlorophyll, transmembrane
• Biological source: Rhodobacter sphaeroides; More
• Total number of polymer chains: 3
• Total formula weight: 100630.18

Authors

Katona, G.,Andreasson, U.,Landau, E.M.,Andreasson, L.-E.,Neutze, R. (deposition date: 2003-05-13, release date: 2003-08-05, Last modification date: 2023-12-13)

Primary citation

Katona, G.,Andreasson, U.,Landau, E.M.,Andreasson, L.-E.,Neutze, R.
Lipidic Cubic Phase Crystal Structure of the Photosynthetic Reaction Centre from Rhodobacter Sphaeroides at 2.35 A Resolution
J.Mol.Biol., 331:681-, 2003

PubMed Abstract: Well-ordered crystals of the bacterial photosynthetic reaction centre from Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we report the type I crystal packing that results from this crystallisation medium, for which 3D crystals grow as stacked 2D crystals, and the reaction centre X-ray structure is refined to 2.35A resolution. In this crystal form, the location of the membrane bilayer could be assigned with confidence. A cardiolipin-binding site is found at the protein-protein interface within the membrane-spanning region, shedding light on the formation of crystal contacts within the membrane. A chloride-binding site was identified in the membrane-spanning region, which suggests a putative site for interaction with the light-harvesting complex I, the cytochrome bc(1) complex or PufX. Comparisons with the X-ray structures of this reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich environment.

PubMed: 12899837
DOI: 10.1016/S0022-2836(03)00751-4

Experimental method

X-RAY DIFFRACTION (2.35 Å)