1OC4

Lactate dehydrogenase from Plasmodium berghei

1OC4 の概要

• エントリーDOI: 10.2210/pdb1oc4/pdb
• 関連するPDBエントリー: 1LDG
• 分子名称: L-LACTATE DEHYDROGENASE, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, OXAMIC ACID, ... (5 entities in total)
• 機能のキーワード: lactate dehydrogenase, oxidoreductase, glycolysis, interconversion of pyruvate and lactate, 3-layer (aba) sandwich
• 由来する生物種: PLASMODIUM BERGHEI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72281.00

構造登録者

Winter, V.J.,Brady, R.L. (登録日: 2003-02-05, 公開日: 2003-09-18, 最終更新日: 2023-12-13)

主引用文献

Winter, V.J.,Cameron, A.,Tranter, R.,Sessions, R.B.,Brady, R.L.
Crystal Structure of Plasmodium Berghei Lactate Dehydrogenase Indicates the Unique Structural Differences of These Enzymes are Shared Across the Plasmodium Genus
Mol.Biochem.Parasitol., 131:1-, 2003

PubMed Abstract: As Plasmodium rely extensively on homolactic fermentation for energy production, Plasmodium falciparum lactate dehydrogenase (PfLDH)--the key enzyme in this process--has previously been suggested as a novel target for antimalarials. This enzyme has distinctive kinetic and structural properties that distinguish it from its human homologues. In this study, we now describe the expression, kinetic characterisation and crystal structure determination of the LDH from Plasmodium berghei. This enzyme is seen to have a similar kinetic profile to its P. falciparum counterpart, exhibiting the characteristic lack of substrate inhibition that distinguishes plasmodial from human LDHs. The crystal structure of P. berghei lactate dehydrogenase (PbLDH) shows a very similar active site arrangement to the P. falciparum enzyme. In particular, an insertion of five amino acid residues in the active site loop creates an enlarged volume in the substrate binding site, and characteristic changes in the residues lining the NADH cofactor binding pocket result in displacement of the cofactor relative to its observed position in mammalian and all other LDH structures. These results imply the special features previously described for PfLDH may be shared across the Plasmodium genus, supporting the universal application of therapeutics targeting this enzyme.

PubMed: 12967707
DOI: 10.1016/S0166-6851(03)00170-1

実験手法

X-RAY DIFFRACTION (2.3 Å)