1O7Y

Crystal structure of IP-10 M-form

Summary for 1O7Y

• Entry DOI: 10.2210/pdb1o7y/pdb
• Related: 1LV9 1O7Z 1O80
• Descriptor: SMALL INDUCIBLE CYTOKINE B10, SULFATE ION (2 entities in total)
• Functional Keywords: chemokine, interferon induction, chemotaxis, inflammatory response
• Biological source: HOMO SAPIENS (HUMAN)
• Total number of polymer chains: 4
• Total formula weight: 34741.51

Authors

Swaminathan, G.J.,Holloway, D.E.,Papageorgiou, A.C.,Acharya, K.R. (deposition date: 2002-11-20, release date: 2003-05-08, Last modification date: 2024-10-23)

Primary citation

Swaminathan, G.J.,Holloway, D.E.,Colvin, R.A.,Campanella, G.K.,Papageorgiou, A.C.,Luster, A.D.,Acharya, K.R.
Crystal Structures of Oligomeric Forms of the Ip-10/Cxcl10 Chemokine
Structure, 11:521-, 2003

PubMed Abstract: We have determined the structure of wild-type IP-10 from three crystal forms. The crystals provide eight separate models of the IP-10 chain, all differing substantially from a monomeric IP-10 variant examined previously by NMR spectroscopy. In each crystal form, IP-10 chains form conventional beta sheet dimers, which, in turn, form a distinct tetrameric assembly. The M form tetramer is reminiscent of platelet factor 4, whereas the T and H forms feature a novel twelve-stranded beta sheet. Analytical ultracentrifugation indicates that, in free solution, IP-10 exists in a monomer-dimer equilibrium with a dissociation constant of 9 microM. We propose that the tetrameric structures may represent species promoted by the binding of glycosaminoglycans. The binding sites for several IP-10-neutralizing mAbs have also been mapped.

PubMed: 12737818
DOI: 10.1016/S0969-2126(03)00070-4

Experimental method

X-RAY DIFFRACTION (3 Å)