1O7Y
Crystal structure of IP-10 M-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-04 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.979, 53.722, 53.366 |
| Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.000 |
| R-factor | 0.267 |
| Rwork | 0.267 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rhp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.096 | 0.434 |
| Total number of observations | 57731 * | |
| Number of reflections | 7390 | |
| <I/σ(I)> | 10.1 | 2.6 |
| Completeness [%] | 95.4 | 92.8 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.4 | 16 * | 10MG/ML PROTEIN, 16% PEG 4000, 0.1M SODIUM ACETATE BUFFER, PH 4.4, 0.2M AMMONIUM SULPHATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | in water |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.4 |
| 3 | 1 | reservoir | PEG4000 | 16 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
