1NZE

Crystal structure of PsbQ polypeptide of photosystem II from higher plants

1NZE の概要

• エントリーDOI: 10.2210/pdb1nze/pdb
• 関連するPDBエントリー: 1FE1 1ILX
• 分子名称: Oxygen-evolving enhancer protein 3, ZINC ION (3 entities in total)
• 機能のキーワード: photosystem ii, oxygen-enhancer evolving complex, water oxidizing complex, psbq, oee3, oxidoreductase
• 由来する生物種: Spinacia oleracea (spinach)
• 細胞内の位置: Plastid, chloroplast thylakoid membrane: P12301
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16610.15

構造登録者

Calderone, V.,Trabucco, M.,Vujicic, A.,Battistutta, R.,Giacometti, G.M.,Andreucci, F.,Barbato, R.,Zanotti, G. (登録日: 2003-02-17, 公開日: 2003-08-26, 最終更新日: 2024-02-14)

主引用文献

Calderone, V.,Trabucco, M.,Vujicic, A.,Battistutta, R.,Giacometti, G.M.,Andreucci, F.,Barbato, R.,Zanotti, G.
Crystal structure of the PsbQ protein of photosystem II from higher plants
Embo Rep., 4:900-905, 2003

PubMed Abstract: The smallest extrinsic polypeptide of the water-oxidizing complex (PsbQ) was extracted and purified from spinach (Spinacia oleracea) photosystem II (PSII) membranes. It was then crystallized in the presence of Zn(2+) and its structure was determined by X-ray diffraction at 1.95-A resolution using the multi-wavelength anomalous diffraction method, with the zinc as the anomalous scatterer. The crystal structure shows that the core of the protein is a four-helix bundle, whereas the amino-terminal portion, which possibly interacts with the photosystem core, is not visible in the crystal. The distribution of positive and negative charges on the protein surface might explain the ability of PsbQ to increase the binding of Cl(-) and Ca(2+) and make them available to PSII.

PubMed: 12949587
DOI: 10.1038/sj.embor.embor923

実験手法

X-RAY DIFFRACTION (1.95 Å)