1NW2

The crystal structure of the mutant R82E of Thioredoxin from Alicyclobacillus acidocaldarius

Summary for 1NW2

• Entry DOI: 10.2210/pdb1nw2/pdb
• Related: 1NSW 1QUW 2TRX
• Descriptor: THIOREDOXIN, ZINC ION, ACETATE ION, ... (5 entities in total)
• Functional Keywords: thermostability, thioredoxin, electron transport
• Biological source: Alicyclobacillus acidocaldarius
• Total number of polymer chains: 8
• Total formula weight: 93851.74

Authors

Bartolucci, S.,De Simone, G.,Galdiero, S.,Improta, R.,Menchise, V.,Pedone, C.,Pedone, E.,Saviano, M. (deposition date: 2003-02-05, release date: 2003-08-05, Last modification date: 2024-10-30)

Primary citation

Bartolucci, S.,De Simone, G.,Galdiero, S.,Improta, R.,Menchise, V.,Pedone, C.,Pedone, E.,Saviano, M.
An integrated structural and computational study of the thermostability of two thioredoxin mutants from Alicyclobacillus acidocaldarius
J.Bacteriol., 185:4285-4289, 2003

PubMed Abstract: We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.

PubMed: 12837806
DOI: 10.1128/JB.185.14.4285-4289.2003

Experimental method

X-RAY DIFFRACTION (1.9 Å)