1NPM

NEUROPSIN, A SERINE PROTEASE EXPRESSED IN THE LIMBIC SYSTEM OF MOUSE BRAIN

1NPM の概要

• エントリーDOI: 10.2210/pdb1npm/pdb
• 分子名称: NEUROPSIN, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: serine proteinase, glycoprotein
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Secreted: Q61955
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49850.51

構造登録者

Kishi, T.,Kato, M.,Shimizu, T.,Kato, K.,Matsumoto, K.,Yoshida, S.,Shiosaka, S.,Hakoshima, T. (登録日: 1998-01-07, 公開日: 1999-03-23, 最終更新日: 2024-10-30)

主引用文献

Kishi, T.,Kato, M.,Shimizu, T.,Kato, K.,Matsumoto, K.,Yoshida, S.,Shiosaka, S.,Hakoshima, T.
Crystal structure of neuropsin, a hippocampal protease involved in kindling epileptogenesis.
J.Biol.Chem., 274:4220-4224, 1999

PubMed Abstract: Neuropsin is a novel serine protease, the expression of which is highly localized in the limbic areas of the mouse brain and which is suggested to be involved in kindling epileptogenesis and hippocampal plasticity. The 2.1-A resolution crystal structure of neuropsin provides the first three-dimensional view of one of the serine proteases highly expressed in the nervous system, and reveals a serine protease fold that exhibits chimeric features between trypsin and nerve growth factor-gamma (NGFgamma), a member of the kallikrein family. Neuropsin possesses an N-glycosylated "kallikrein loop" but forms six disulfide bonds corresponding to those of trypsin. The ordered kallikrein loop projects proline toward the active site to restrict smaller residues or proline at the P2 position of substrates. Loop F, which participates in forming the S3/S4 sites, is similar to trypsin rather than NGFgamma. The unique conformations of loops G and H form an S1 pocket specific for both arginine and lysine. These characteristic loop structures forming the substrate-binding site suggest the novel substrate specificity of neuropsin and give a clue to the design of its specific inhibitors.

PubMed: 9933620
DOI: 10.1074/jbc.274.7.4220

実験手法

X-RAY DIFFRACTION (2.1 Å)