1NMM

beta-1,4-galactosyltransferase mutant Cys342Thr complex with alpha-lactalbumin and GlcNAc

Replaces:  1JN8

Summary for 1NMM

Related1J92
DescriptorALPHA-LACTALBUMIN, BETA-1,4-GALACTOSYLTRANSFERASE, CALCIUM ION, ... (6 entities in total)
Functional Keywordsbeta1, 4-galactosyltransferase, cys342thr mutation, alpha-lactalbumin complex, transferase activator-transferase complex, transferase activator/transferase
Biological sourceMus musculus (house mouse)
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Cellular locationSecreted P29752
Isoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Isoform Short: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Processed beta-1,4-galactosyltransferase 1: Secreted P08037
Total number of polymer chains4
Total molecular weight94641.85
Authors
Ramakrishnan, B.,Shah, P.S.,Qasba, P.K. (deposition date: 2003-01-10, release date: 2003-01-21, Last modification date: 2011-07-13)
Primary citation
Ramakrishnan, B.,Shah, P.S.,Qasba, P.K.
alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc.
J.Biol.Chem., 276:37665-37671, 2001
PubMed: 11485999 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M102458200
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.220110.3%5.2%4.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution