1NGW

Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin

1NGW の概要

• エントリーDOI: 10.2210/pdb1ngw/pdb
• 関連するPDBエントリー: 1N7M 3FCT
• 分子名称: Mature Metal Chelatase Catalytic Antibody, Light chain, Mature Metal Chelatase Catalytic Antibody, Heavy chain, N-METHYLMESOPORPHYRIN, ... (4 entities in total)
• 機能のキーワード: antibody, immunoglobulin, antigen binding fragment, immune system
• 由来する生物種: Mus musculus, Homo sapiens (house mouse, human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94219.61

構造登録者

Yin, J.,Andryski, S.E.,Beuscher IV, A.E.,Stevens, R.C.,Schultz, P.G. (登録日: 2002-12-18, 公開日: 2003-02-04, 最終更新日: 2024-10-30)

主引用文献

Yin, J.,Andryski, S.E.,Beuscher IV, A.E.,Stevens, R.C.,Schultz, P.G.
Structural evidence for substrate strain in antibody catalysis
Proc.Natl.Acad.Sci.USA, 100:856-861, 2003

PubMed Abstract: The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.

PubMed: 12552112
DOI: 10.1073/pnas.0235873100

実験手法

X-RAY DIFFRACTION (2.6 Å)