1NGW
Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.721, 134.215, 72.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.600 |
| Rwork | 0.275 |
| R-free | 0.30000 * |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 2.600 * | |
| Rmerge | 0.108 * | 0.672 * |
| Total number of observations | 140870 * | |
| Number of reflections | 28954 * | |
| Completeness [%] | 98.7 * | 97.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 * | 25 * | PEG2000MME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG2000 MME | 27 (%) | |
| 2 | 1 | drop | ammonium sulfate | 250 (mM) | |
| 3 | 1 | drop | MP | 0.5 (mM) | |
| 4 | 1 | drop | HEPES | 10 (mM) | pH6.6 |
| 5 | 1 | reservoir | PEG2000MME | 27 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 7 | 1 | reservoir | Tris | 100 (mM) | pH8.6 |
