1NFK

STRUCTURE OF THE NUCLEAR FACTOR KAPPA-B (NF-KB) P50 HOMODIMER

Summary for 1NFK

• Entry DOI: 10.2210/pdb1nfk/pdb
• Descriptor: DNA (5'-D(*TP*GP*GP*GP*AP*AP*TP*TP*CP*CP*C)-3'), PROTEIN (NUCLEAR FACTOR KAPPA-B (NF-KB)) (3 entities in total)
• Functional Keywords: nf-kb p50, complex (transcription factor-dna), transcription-dna complex, transcription/dna
• Biological source: Mus musculus (house mouse)
• Cellular location: Nucleus. Isoform 5: Cytoplasm. Isoform 6: Nucleus. Isoform 7: Nucleus: P25799
• Total number of polymer chains: 4
• Total formula weight: 79894.07

Authors

Ghosh, G.,Van Duyne, G.,Ghosh, S.,Sigler, P.B. (deposition date: 1995-02-28, release date: 1996-12-23, Last modification date: 2024-11-13)

Primary citation

Ghosh, G.,van Duyne, G.,Ghosh, S.,Sigler, P.B.
Structure of NF-kappa B p50 homodimer bound to a kappa B site.
Nature, 373:303-310, 1995

PubMed Abstract: The 2.3-A crystal structure of the transcription factor NK-kappa B p50 homodimer bound to a palindromic kappa B site reveals that the Rel homology region folds into two distinct domains, similar to those in the immunoglobulin superfamily. The p50 dimer envelopes an undistorted B-DNA helix, making specific contacts along the 10-base-pair kappa B recognition site mainly through loops connecting secondary structure elements in both domains. The carboxy-terminal domains form a dimerization interface between beta-sheets using residues that are strongly conserved in the Rel family.

PubMed: 7530332
DOI: 10.1038/373303a0

Experimental method

X-RAY DIFFRACTION (2.3 Å)