1NFK
STRUCTURE OF THE NUCLEAR FACTOR KAPPA-B (NF-KB) P50 HOMODIMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.200, 132.100, 80.100 |
| Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.34000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.068 * |
| Number of reflections | 30826 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | ||
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 8 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 11 | 1 | reservoir | ammonium chloride | 100 (mM) | |
| 12 | 1 | reservoir | 10 (mM) | ||
| 13 | 1 | reservoir | spermine | 0.10 (mM) | |
| 14 | 1 | reservoir | beta-octylglucoside | 0.10 (%(w/v)) | |
| 15 | 1 | reservoir | PEG4000 | 8 (%) | |
| 2 | 1 | drop | HEPES | 25 (mM) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | ammonium chloride | 50 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | spermine | 0.05 (mM) | |
| 7 | 1 | drop | beta-octylglucoside | 0.05 (%(w/v)) | |
| 8 | 1 | drop | PEG4000 | 4 (%) | |
| 9 | 1 | reservoir | HEPES | 50 (mM) |
